Composite

Part:BBa_K2680403:Design

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G T500_noGap, short attachment (T11)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 102


Design Notes

Sequence from Murray Lab at California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

Intrinsic terminator "derived from bacteriophage Φ82."1

References

1Greenblatt, Jack. F. (2008). Preview: Transcription termination: pulling out al the stops. Cell, 132, 918. doi:10.1016/j.cell.2008.03.003 Sequence and Features